CK-MB is an enzyme present in the cardiac muscle, with a molecular weight of 87.0 kda. Creatine kinase (CK) is a dimeric molecule formed from two subunits designated as "m" and "b", which combine to form three different isoenzymes, ck-mm, ck-bb and ck-MB. Ck-MB is the isoenzymes of creatine kinase most involved in the metabolism of cardiac muscle tissue. The release of ck-MB into the blood following a myocardial infarction can be detected within 3-8 hours after the onset of symptoms. It peaks within 9-30 hours, and returns to baseline levels within 48 to 72 hours.
One step CK-MB test is a simple test that utilizes a combination of anti-ckmb antibody coated particles and capture reagent to selectively detect ckmb in whole blood, serum or plasma or plasma. The minimum detection level is 7ng/ml.